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KMID : 0613820040140010193
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Journal of Life Science
2004 Volume.14 No. 1 p.193 ~ p.197
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Antifungal Compound Produced by Bacillus sp. TBM912
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Joo Woo-Hong
Han Su-Ji
Choi Yong-Lark
Jeong Young-Kee
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Abstract
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A continuous enrichment culture procedure was used to isolate bacteria from various soil sources capable of suppressing large patch disease of turfgrass. Six isolates consistently suppressed large patch in turfgrass, and ranged in the spectrum of extracellular enzymes that they expressed. The best disease- suppressing isolate, TBM912, expressed protease, CMCase, and pectinase activity and inhibited the growth of Rhizoctonia solani and Botrytis cinerea in vitro. Here we show that this strain also produces an antibiotic that was identified by TLC, SDS-PAGE and HPLC analysis as lipopeptide.
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KEYWORD
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Bacillus sp., antifungal compound, Rhizoctonia solani, Botrytis cinerea, Disease-suppression
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